Isolation and Physico-chemical Characterization of Quercetin from the Ethanolic Extract of Syrian Hypericum perforatum L. Plant as Antibacterial
Hadeel Mosalem *
Department of Chemistry, Faculty of Science, Tishreen University, Latakia, Syria.
Nizar Maalla
Department of Crops, Faculty of Agriculture, Tishreen University, Latakia, Syria.
Yaser Hammad
Department of Soil, Faculty of Agriculture, Tishreen University, Latakia, Syria.
Emad Hwije
Department of Chemistry, Faculty of Science, Tishreen University, Latakia, Syria.
*Author to whom correspondence should be addressed.
Abstract
Quercetin compound was isolated from the ethanolic extract of the flowers of the Syrian Hypericum perforatum L. plant, which was collected from the Al-Qadmus region in Syria (at an altitude of 900 meters), using column chromatography and thin layer technology. The identity of the isolated compound was confirmed using (IR, 1HNMR, 13CNMR) spectroscopy.
The effectiveness of the organic extract and the compound isolated from it was also tested against a Gram-positive bacterial strain (Staphylococcus aureus) and a Gram-negative bacterial strain (Escherichia coli) at concentrations of the extract:(5,10, 20)%, and the following quantities of the isolated compound (5,10,20µl).
The results showed that the best mobile phase for isolating quercetin from the column was (hexane ethyl acetate - ethanol) (1:2:1), then in thin layers with a displacement mixture (hexane - dichloromethane) (1:2), and it was valuable RF= 0.45.
And results also showed better effectiveness of quercetin in quantity(20µl) than the effectiveness of the ethanolic extract isolated from it at all concentrations used against the two bacterial strains mentioned previously with two levels of inhibition 21.36mm, 8.77mm for each Staph. aureus, E. coli, respectively
Keywords: Integrated nutrient management, Hypericum perforatum L, mulberry, ethanolic extract, leaf quality, quercetin, bioassay, IR, 1HNMR, 13CNMR, Staphylococcus aureus, Escherichia coli, Antibiotics