Asian Journal of Advances in Research

The green synthesis of nano-sized particles with specific functions is of great significance in the present bionanotechnology. In this study, the biosynthesis of silver nanoparticles from the aqueous leaf extract of Rhizophora stylosa, mangrove plant from southern India, and its activity against some bacterial pathogens are reported for the first time. A green procedure for the synthesis of silver nanoparticles using the mangrove extract as reducing agent was used. Synthesized silver nanoparticles were investigated using UV–visible spectrophotometry, Scanning electron microscope, Transmission electron microscope, X-ray diffraction, Selected area electron diffraction and Fourier transform infrared spectroscopy. The biosynthesized silver nanoparticles were characterized by UV–visible spectrophotometry at a wavelength of 422 nm confirmed the synthesis of silver nanoparticles. The results from the X-ray diffraction analysis approved the formation of crystalline silver nanoparticles with crystallinity percentage of 85.87. As shown by Transmission electron microscope evaluation, silver nanoparticles had the same spherical morphology. The size of the biosynthesized silver nanoparticles was between 1 and 75 nm with average size of 38.62 nm. Scanning electron microscope images identified silver nanoparticles ranging in size from 18 to 42 nm. Fourier transform infrared spectroscopy analysis distinguished different functional groups such as aromatic loops, alcohol, phenol group, alkanes and alkyl halides in the biosynthesis process. Green biosynthesis of silver nanoparticles using aqueous extract of mangrove R. stylosa appears rapid, reliable, nontoxic, and eco-friendly.