Asian Journal of Advances in Research

Recent advancements in nanotechnology based compounds have opened a new horizon for combating multi drug resistance in microorganisms. In particular, the use of silver nanoparticles as a potent for antibacterial agent has focused much attention. Silver nanoparticles exhibit their antimicrobial abilities through multifaceted mechanisms. Silver nanoparticles adhesive to microbial cells, penetration inside the cells, and modulation of the microbial signal transduction pathways have been recognized as the most empirical mode of antimicrobial action. Therefore, an attempt was made to produce silver nanoparticles having physicochemical properties. Thus, eco-friendly, non-toxic silver nanoparticles were green synthesized using the leaf extract of Lumnitzera racemosa and its phytochemical screening & antibacterial activity were carried out against clinical pathogens. The synthesis of silver nanoparticles was confirmed by a change in extract color from dark green to brown and surface Plasmon resonance spectra obtained at the range of approximately 537 nm. The antibacterial activity of the green synthesized silver nanoparticles against bacterial strains such as Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumoniae using well diffusion method. The results of antimicrobial activity by the well diffusion assay also clearly expressed that test extract have high concentration of active principles. The aqueous leaf extract of Lumnitzera racemosa shows the presence of   phytochemical   compounds   such   as   alkaloids,   flavonoids,   glycosides,   phenols,   saponins, tannins   and   terpenoids.