BIOGENIC SYNTHESIS OF SILVER NANOPARTICLES FROM MANGROVE PLANT Lumnitzera racemosa AND ITS PHYTOCHEMICAL SCREENING & ANTIBACTERIAL ACTIVITY
M. L. MOHAMMED KALEEM ARSHAN *
Department of Biotechnology, Islamiah College (Autonomous), Vaniyambadi, India.
S. IMADUDDIN
Department of Biochemistry, Madras University, Chennai, India.
FARHANAAZ MAGI
Department of Botany, Secab ARS Inamdar Arts, Science and Commerce Degree College for Women, Vijayapur, India.
*Author to whom correspondence should be addressed.
Abstract
Recent advancements in nanotechnology based compounds have opened a new horizon for combating multi drug resistance in microorganisms. In particular, the use of silver nanoparticles as a potent for antibacterial agent has focused much attention. Silver nanoparticles exhibit their antimicrobial abilities through multifaceted mechanisms. Silver nanoparticles adhesive to microbial cells, penetration inside the cells, and modulation of the microbial signal transduction pathways have been recognized as the most empirical mode of antimicrobial action. Therefore, an attempt was made to produce silver nanoparticles having physicochemical properties. Thus, eco-friendly, non-toxic silver nanoparticles were green synthesized using the leaf extract of Lumnitzera racemosa and its phytochemical screening & antibacterial activity were carried out against clinical pathogens. The synthesis of silver nanoparticles was confirmed by a change in extract color from dark green to brown and surface Plasmon resonance spectra obtained at the range of approximately 537 nm. The antibacterial activity of the green synthesized silver nanoparticles against bacterial strains such as Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumoniae using well diffusion method. The results of antimicrobial activity by the well diffusion assay also clearly expressed that test extract have high concentration of active principles. The aqueous leaf extract of Lumnitzera racemosa shows the presence of phytochemical compounds such as alkaloids, flavonoids, glycosides, phenols, saponins, tannins and terpenoids.
Keywords: Allornetric studies,, Silver nanoparticles, teleost brain, mangrove plant, Lumnitzera racemosa, phytochemical screening and antibacterial activity.
How to Cite
Downloads
References
Faisal N, Kumar K. Polymer and metal nanocomposites in biomedical applications. Biointerface Res. Appl. Chem. 2017;7:2286–2294.
Alexander JW. History of the medical use of silver. Surg. Infect. 2009;10:289–292.
Ioan-Avram N, Anton F, Maria S, Denisa F, Ovidiu O, Ecaterina A. Silver based materials for biomedical applications. Curr. Org. Chem. 2014;18:173–184.
Geraldo DA, Needham P, Chandia N, Arratia-Perez R, Mora GC, Villagra NA. Green synthesis of polysaccharides-based gold and silver nanoparticles and their promissory biological activity. Biointerface Res. Appl. Chem. 2016;6:1263–1271.
Chowdhury NR, MacGregor-Ramiasa M, Zilm P, Majewski P, Vasilev K. ‘Chocolate’ silver nanoparticles: Synthesis, antibacterial activity and cytotoxicity. J. Colloid Interface Sci. 2016;482:151–158.
Wei L, Lu J, Xu H, Patel A, Chen ZS, Chen G. Silver nanoparticles: Synthesis, properties, and therapeutic applications. Drug Discov. Today. 2015;20:595–601.
Sangeetha A, Saraswathi U, Singaravelu GJ. Green synthesis of silver nanoparticles using a mangrove Excoecaria agallocha, Int. J. Pharm. Sci. Invent. 2014;3:54–57.
Bakshi M, Ghosh S, Chaudhuri P. Green synthesis, characterization and antimicrobial potential of sliver nanoparticles using three mangrove plants from Indian Sundarban, BioNanoScience. 2015;5:162–170.
Balakrishnan S, Srinivasan M, Mohanraj J. Biosynthesis of silver nanoparticles from mangrove plant (Avicennia marina) extract and their potential mosquito larvicidal property, J. Parasit. Dis. 2016;40:991–996.
Gnanadesigan M, Anand M, Ravikumar S, Maruthupandy M, Syed Ali M, Vijayakumar V, Kumaraguru AK. Antibacterial potential of biosynthesised silver nanoparticles using Avicennia marina mangrove plant, Appl. Nanosci. 2012;2:143–147.
Abdi V, Sourinejad I, Yousefzadi M, Ghasemi Z. Mangrove-mediated synthesis of silver nanoparticles using native Avicennia marina plant extract from southern Iran. Chemical Engineering Communications. 2018;205(8): 1069–1076.
Umashankari J, Inbakandan D, Ajithkumar TT, Balasubramanian T. Mangrove plant, Rhizophora mucronata (Lamk, 1804) mediated one pot green synthesis of silver nanoparticles and its antibacterial activity against aquatic pathogens, Aquat. Biosyst. 2012;8:11.
Gnanadesigan M, Anand M, Ravikumar S, Maruthupandy M, Vijayakumar V, Selvam S, Dhineshkumar M, Kumaraguru AK. Biosynthesis of silver nanoparticles by using mangrove plant extract and their potential mosquito larvicidal property, Asian Pac. J. Trop. Med. 2011;4:799–803.
Abdi V, Sourinejad I, Yousefzadi M, Ghasemi Z. Biosynthesis of Silver Nanoparticles from the Mangrove Rhizophora mucronata: Its Characterization and Antibacterial Potential. Iranian Journal of Science and Technology, Transactions A: Science; 2019.
Panigrahi T. Synthesis and characterization of silver nanoparticles using leaf extract of Azadirachta indica. Dep Life Sci Natl Inst Technol Rourkela (Doctoral dissertation); 2013.
Niraimathi KL, Sudha V, Lavanya R, Brindha P. BiointerfacesBiosynthesis of silver nanoparticles using Alternanthera sessilis (Linn.) extract and their antimicrobial, antioxidant activities. Colloids and Surfaces B: Biointerfaces. 2013;102:288-291.32.
Oluwaniyi OO, Adegoke HI, Adesuji ET, Alabi AB, Bodede SO, et al. Biosynthesis of silver nanoparticles using aqueous leaf extract of Thevetia peruviana Juss and its antimicrobial activities. Appl Nanosci. 2015;6:903-912.33.
Ganesh J, Jannet Vennila. Phytochemical Analysis of Acanthus ilicifolius and Avicennia officinalis By GC-MS. Research Journal of Phytochemistry. 2011;5:60-65.
Patra JK, Panigrahi TK, Rath SK, Dhal NK, Thatoi H. Phytochemical screening and antimicrobial assessment of leaf extracts of Exoecaria agallocha L.: A mangal species of Bhitarkanika, Orissa, India. Adv Ind Nat Appl Sci. 2009;3:241–6.
Aboaba OO, Smith SI, Olude FO. Antimicrobial effect of edible plants extracts on Escherichia coli1057: H7. Pak. J. Nutr. 2006;5(4):325-327.
Okwu DE. Phytochemicals and vitamin content of indigenous species of South Eastern Nigeria. J. Sustainable Agri. Environ. 2004;6(1):30-37.
Okwu DE. Evaluation of the chemical composition of indigenous spices and flavouring Agents. Global J. Pure Appl. Sci. 2001;7(3):455-459.
Salah N, Miller NJ, Pagange G, Tijburg L, Bolwell GP, Rice E, Evans C. Polyphenolic flavonoids as scavenger of aqueous phase radicals as chain breaking antioxidant. Arc. Biochem. Broph. 1995;2: 339−346.
Okwu DE, Okwu ME. Chemical composition of Spondias mombin linn. Plant parts. J. Sustain. Agric. Environ. 2004;6:140−147.
Ali SS, Kasoju N, Luthra A, Singh A, Sharanabasava H, Sahuand A, Bora U. Indian medicinal herbs as source of antioxidants. Food Res. Int. 2008;41:1-15.
Sodipo OA, Akiniyi JA, Ogunbamosu JU. Studies oncertain on certain characteristics of extracts of bark of Pansinystalia macruceras (K schemp) picrre Exbeille. Global J.Pure Appl. Sci. 2000;6:83-87.
Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: A case study on E. coli as a model for Gram-negative bacteria. J. Colloid Interface Sci. 2004;275:177–182.
Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramírez JT, et al. The bactericidal effect of silver nanoparticles. Nanotechnology. 2005;16:2346–2353.