PHYTOCHEMICAL SCREENING AND ANALYTICAL ASSESSMENT OF ACID-BASE INDICATOR PROPERTIES OF RED AND WHITE KOLA NUTS EXTRACTS
ANIEKAN E. AKPAKPAN *
Akwa Ibom State University, Ikot Akpaden, Nigeria.
EMAIME J. UWANTA
Akwa Ibom State University, Ikot Akpaden, Nigeria.
PATRICK E. AKPAN
Akwa Ibom State Polytechnic, Ikot Osurua, Nigeria.
ANIEKAN E. OWEN
Akwa Ibom State University, Ikot Akpaden, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
In Nigeria, the most commonly used indicator for acid-base titration is synthetic indicator, which is non biodegradable when exposed to the environment. The need to develop acid-base indicators from natural sources to serve as an effective alternative for synthetic indicators is the demand for environment green chemistry. This research work is aimed at the extraction, phytochemical screening, UV/visible spectroscopic analysis of different extracts of red and white kola nuts and application of the extracts as eco-friendly, cost effective and sustainable indicators for acid- base titrations. Extraction was done for 24 hours using acetone, ethanol, cold and hot water separately. The results revealed that all the extracts contained varying amount of phytochemicals, except anthraquinone and phlobatannins which were absents in all the extracts. UV/visible spectroscopic analysis carried out at 600 nm showed that all the extracts have varying absorbance in acidic (HCl) and basic (NaOH) mediums. The potentials of the acid-base indicator of the extracts were evaluated using the titrimetric reactions of strong acid/strong base (HCl/NaOH), strong acid/week base (HCl/NH4OH), week acid/strong base (CH3COOH /NaOH) and week acid/week base (CH3COOH/ NH4OH). The results revealed that the extracts of red kola nut give remarkable colour changes in the titration of strong acid/strong base and strong acid/ weak base. While white kola nut extracts showed remarkable colour changes for all titrations, except in the titration of weak acid with strong base using ethanol and hot water extracts. However, kola nuts extracts which are non toxic, environmentally friendly and biodegradable can serve as effective alternative to synthetic indicator for acid-base titrations.
Keywords: Acid- base titration, kola nuts extracts, indicator, phytochemicals
How to Cite
References
Arif Khan PM, Farooqui M. Analytical applications of plant extracts as natural ph indicator: A review. Journal of Advanced Scientific Research. 2012;2(4):20-27.
Eze SO, Ogbuefi RA. Analytical potentials of dye extract from Aspilia africana (Oramejula) flowers. Asia Journal of Natural Products and Applied Sciences. 2014;3(1):54-60.
Onwuachu UI, Aboh UA, Iwuoha G. Extraction, characterization and workability of some local plant dyes as acid-base indicators. International Journal of Research in Applied, Natural and Social Sciences. 2014;2(7):1-6.
Okoduwa Stanley IR, Mbora Lovina O, Adu Matthew E, Adeyi Ameh A. Comparative analysis of the properties of acid-base indicator of rose (Rosa setigera), allamanda (Allamanda cathartica) and hibiscus (Hibiscus rosa-sinensis) flowers. Hindawi Publishing Corporation Biochemistry Research International. 2015;1-6.
Atkins PW. Anthocyanin pigment for sour cherries. Journal of American Chemical Society. 1987;78:979.
Bhise SH, Shinde NG, Surve BS, Pimpodkar NV, Shikalgar SS. Acalypha wilkesiana as natural pH indicator. International Journal of Natural Products Research. 2014;4(1):33- 35.
Jaspreet S, Kanika A, Parminder N, Geeta D. Herbal indicators as an upcoming trend in titrimetric analysis. International Research Journal of Pharmacy. 2011;2(4):177-179.
Abbas SK. Study of acid-base indicator property of flowers of Ipomoea biloba. International Current Pharmaceutical Journal. 2012;1(12):420-422.
Gupta P, Jain P, Jain PK. Dahalia flower sap a natural resource as indicator in acidimetry and alkalimetry. Int. J. Pharm. Tech. 2013;4(4):5038-5045.
Daniel Abugri A, Ohene Apea B, Gregory Pritchett. Investigation of a simple and cheap source of a natural indicator for acid-base titration: Effects of system conditions on natural indicators. Green and Sustainable Chemistry. 2012;2:117-122.
Izonfuo WAL, Fekarurhobo GK, Obomanu FG, Daworiye LT. Acid-base indicator properties of dyes from local plants: Dyes from Basella alba (Indian spinach) and Hibiscus sabdariffa (Zobo). Journal of Applied Sciences and Environmental Management. 2006;1(1):5-8.
Odiongenyi AO, Akpakpan AE, Enengedi IS, Ekpo EI. Evaluation of potentials of Dissotis rotundifolia, Centrosema pubescens and Allium cepa extracts as indicator for acid-base titration. International Journal of Advanced and Innovative Research. 2016;5(3):2278-7844.
Burdock GA, Carabin IG, Crincoli CM. Safety assessment of kola nut extract as a food ingredient. Food and Chemical Toxicology. 2009;47(8):1725-32.
Voelcker OJ. Cotyledon colour in kola. Tropical Agriculture. 1935;12:231-234.
Odebunmi EO, Oluwaniyi OO, Awolola GV, Adediji OD. Proximate and nutritional composition of kola nut (Cola nitida), bitter cola (Garcinia cola) and alligator pepper (Afromomum melegueta). African Journal of Biotechnology. 2009;8(2):308-310.
Dewole EA, Dewumi DF, Alabi JY, Adegoke A. Proximate and phytochemical analysis of Cola nitida and Cola acuminata. Pakistan Journal of Biological Sciences. 2013;16(22):1593-1596.
Stanley Okoduwa IR, Lovina Mbora O, Matthew Adu E, Ameh A. Adeyi. Comparative analysis of the properties of acid-base indicator of rose (Rosa setigera), allamanda (Allamanda cathartica) and hibiscus (Hibiscus rosa-sinensis) flowers. Hindawi Publishing Corporation Biochemistry Research International. 2015;1-6.
Shabi Ruskin R, Vasantha Kumari Chitarasu. Qualitative phytochemical characterization of thorn extracts of Canthium parviflorum Lam. Int. Res J. Pharm. App Sci. 2014;4(5):13-17.
Sawant RS, Godghate AG. Qualitative phytochemical screening of rhizomes of Curcuma longa Linn. International Journal of Science, Environment and Technology. 2013;2(4):634-641.
Patil SB, Kondawar MS, Ghodke DS, Naikwade NS, Magdum CS. Use of flower extracts as an indicator in acid-base titrations. Research J. Pharm. and Tech. 2009;2(2):421-422.
Agarwal OP. Chemistry of organic natural products. Vol II. Goel Publishing House, Krishana Prakashan Media (p) Ltd., Meerut. 2013;210-213.
Uche Onwuachu I, Uju Aboh A, Godson Iwuoha. Extraction, characterization and workability of some local plant dyes as acid-base indicators. International Journal of Research in Applied, Natural and Social Sciences. 2014;2(7):2347-4580.