Establishment and Verification of a First-Order Derivative UV Spectrophotometric Technique for Quantifying Favipiravir in Bulk and Pharmaceutical Formulations
Thippavati Jayasree
Department of Pharmaceutical Analysis, Vishnu Institute of Pharmaceutical Education and Research, Narsapur, Medak-502313, Telangana, India.
Madapathi Keerthana
Department of Pharmaceutical Analysis, Vishnu Institute of Pharmaceutical Education and Research, Narsapur, Medak-502313, Telangana, India.
Papagari Krishna Vamshi Reddy
Department of Pharmaceutical Analysis, Vishnu Institute of Pharmaceutical Education and Research, Narsapur, Medak-502313, Telangana, India.
Kappala Swetha
Department of Pharmaceutical Analysis, Vishnu Institute of Pharmaceutical Education and Research, Narsapur, Medak-502313, Telangana, India.
Jahnavi Bandla *
Department of Pharmaceutical Analysis, Vishnu Institute of Pharmaceutical Education and Research, Narsapur, Medak-502313, Telangana, India.
*Author to whom correspondence should be addressed.
Abstract
The primary aim of this study was to establish and validate a UV spectrophotometric method for quantifying Favipiravir in both bulk and pharmaceutical formulations using a first-order derivative technique. In this approach, acetonitrile was chosen as the solvent, and measurements were taken at a wavelength of 323 nm. The methodology was developed and subsequently validated in compliance with the ICH guidelines. The analysis revealed a strong linear response within a concentration range of 5-25 µg/ml, with a correlation coefficient of 0.9995. Furthermore, the method exhibited a low detection limit of approximately 0.48 µg/ml and a quantification limit of about 1.44 µg/ml. A comprehensive assessment of the method's precision, accuracy, specificity, and sensitivity produced consistently positive results. Given its robust and reliable performance, this technique can be effectively utilized for the routine analysis of Favipiravir in both bulk and pharmaceutical forms.
Keywords: Favipiravir, UV spectrophotometry, acetonitrile, method development, validation