GC-MS ANALYSIS OF METHANOLIC STEM BARK EXTRACT OF Indigofera zollingeriana
J. O. ALAGBE *
Department of Animal Nutrition and Biochemistry, Sumitra Research Institute, Gujarat, India.
M. D. SHITTU
Department of Animal Production and Health, Ladoke Akintola University of Technology, Ogbomosho, Oyo State, Nigeria.
F. T. USHIE
Department of Agricultural Technology, Federal College of Forestry Mechanization, Afaka Kaduna State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
The present study was carried out to examine the bioactive chemicals in methanolic stem bark extract of Indigofera zollingeriana using Gas Chromatography – Mass Spectrum (GC-MS). The result revealed the presence of 19 compounds with peak percentage in chromatogram. The major phytoconstituent in the exatract are: Indigoferabietone (21.67 %), Indigo, 2, 2-bisindole (14.35 %) and Indigoidin (12.49 %) with retention time of 17.38 min, 20.57 min and 10.33 min respectively. It was concluded that the presence of various bioactive chemicals or phytochemicals in Indigofera zollingeriana stem bark justifies that it can be used for the treatment of many diseases in animals and could be used as an alternative to antibiotics to promote food safety.
Keywords: GC-MS analysis, Indigofera zollingeriana stem bark, methanol, phytochemicals