THE PREVALENCE AND ANTIMICROBIAL RESISTANCE OF Staphylococcus aureus ISOLATED FROM MASTITIS GOAT MILK ADMITTED AT SAQ TEACHING VETERINARY HOSPITAL, BANGLADESH
MD. HAFIZAR RAHMAN
Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Khulshi, Chattogram-4225, Bangladesh.
MOHAMMAD MEJBAH UDDIN
Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Khulshi, Chattogram-4225, Bangladesh.
MD. ZOHORUL ISLAM
Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Khulshi, Chattogram-4225, Bangladesh.
EAFTEKHAR AHMED RANA
Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Khulshi, Chattogram-4225, Bangladesh.
MD. SHOHEL AL FARUK
*
Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Khulshi, Chattogram-4225, Bangladesh.
*Author to whom correspondence should be addressed.
Abstract
Staphylococcus aureus is an infectious, opportunistic organism that causes clinical or subclinical mastitis in dairy goats. The genetic situations and antimicrobial resistance of isolates from dairy goats that are affected in clinical mastitis have not been studied in SAQ Teaching Veterinary Hospital (SAQTVH). The objective of this study was to characterize S. aureus from dairy goats that suffered clinical mastitis, by PCR, and by assessment of antimicrobial susceptibility. A total of 15 S. aureus isolates from intramammary infections were studied. Conventional PCR was used to detect nuc genes. Antimicrobial susceptibility was determined by the using disc diffusion method was carried out. In total 25 (78.12%) samples out of 32 were found Staphylococcus positive according to their cultural properties on Mannitol salt agar and Blood agar, a morphological appearance by Gram’s staining, and 15 (46.87%) samples were found positive by Catalase test. Out of 15 (46.87%) Staphylococcus positive isolates, 3 (20%) samples were Coagulase positive. Among the Coagulase-positive Staphylococcus species, one was confirmed as S. aureus by nuc gene PCR. The remaining isolated Staphylococcus were not confirmed at the species level that was considered unclassified Staphylococcus species. Methicillin resistance was not found, but resistance to penicillin/ ampicillin (81.25%) and (75%) was very common. We found a high degree of relatedness among bovine S. aureus isolates in SAQTVH and the strains that are often resistant to commonly used antimicrobials. This highlights the need for effective preventive measures that aim at limiting the transmission of bacteria rather than using antimicrobials to control S. aureus mastitis.
Keywords: AIDS, Staphylococcus aureus, epidemiology, nuc gene, counselling, antimicrobial resistance, dietary management, mastitis
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