In vitro STUDIES ON CHARACTERIZATION OF POLYHYDROXYBUTYRATE
R. RAMESHWARI *
Cauvery College for Women (Autonomous), Affliated to Bharathidasan University, Trichy, India.
*Author to whom correspondence should be addressed.
Abstract
The bacterial polyesters may be considered as “Green Plastics”, because of their biodegradable nature. These polyesters can be employed for packaging and coating materials / as biodegradable carriers and applied in the biomedical field. Twelve bacterial isolates were isolated from the dairy industry effluent sample. Screening for polyhydroxybutyrate (PHB) was done by Sudan black staining. PHB extraction was carried out by the chloroform digestion method. Biochemical and 16s rRNA analysis showed that PHB producing bacteria belong to Bacillus genera with Maximum production of PHB was analyzed by U.V spectrophotometer and finally it was characterized by FTIR, NMR, and GC MS.
Keywords: Mulberry varieties, Biopolymer, biochemical constituents, polyhydroxybutyrate (PHB), Bombyx mori, dairy industry effluent., bioassay studies