In vitro STUDIES ON CHARACTERIZATION OF POLYHYDROXYBUTYRATE
R. RAMESHWARI *
Cauvery College for Women (Autonomous), Affliated to Bharathidasan University, Trichy, India.
*Author to whom correspondence should be addressed.
Abstract
The bacterial polyesters may be considered as “Green Plastics”, because of their biodegradable nature. These polyesters can be employed for packaging and coating materials / as biodegradable carriers and applied in the biomedical field. Twelve bacterial isolates were isolated from the dairy industry effluent sample. Screening for polyhydroxybutyrate (PHB) was done by Sudan black staining. PHB extraction was carried out by the chloroform digestion method. Biochemical and 16s rRNA analysis showed that PHB producing bacteria belong to Bacillus genera with Maximum production of PHB was analyzed by U.V spectrophotometer and finally it was characterized by FTIR, NMR, and GC MS.
Keywords: Mulberry varieties, Biopolymer, biochemical constituents, polyhydroxybutyrate (PHB), Bombyx mori, dairy industry effluent., bioassay studies
How to Cite
Downloads
References
Chhonkar PK, Datta SP, Joshi HC, Pathak H. Impact of industrial effluents on soil health and agriculture-Indian experience: Part I-Distillery and paper mill effluents; 2000.
Rodrigues MAS, Amado FDR, Xavier JLN, Streit KF, Bernardes AM, Ferreira JZ. Application of photoelectrochemical–electrodialysis treatment for the recovery and reuse of water from tannery effluents. Journal of Cleaner Production. 2008;16(5):605-611.
Verheijen LAHM, Wiersema D, Pol LH, De Wit J. Management of waste from animal product processing. International Agriculture Center, Wageningen, The Netherlands; 1996.
Ganapathy SG, Baskaran R, Mohan PM. Microbial diversity and bioremediation of distilleries effluent. J Res Biol. 2011;3:153-162.
Kushwaha JP, Srivastava VC, Mall ID. An overview of various technologies for the treatment of dairy wastewaters. Critical Reviews in Food Science and Nutrition. 2011; 51(5):442-452.
Perelo LW. In situ and bioremediation of organic pollutants in aquatic sediments. Journal of Hazardous Materials. 2010;177(1-3):81-89.
Porwal HJ, Mane AV, Velhal SG. Biodegradation of dairy effluent by using microbial isolates obtained from activated sludge. Water Resources and Industry. 2015;9: 1-15.
Semrany S, Favier L, Djelal H, Taha S, Amrane A. Bioaugmentation: possible solution in the treatment of bio-refractory organic compounds (Bio-ROCs). Biochemical Engineering Journal. 2012;69:75-86.
Karamalidis AK, Evangelou AC, Karabika E, Koukkou AI, Drainas C, Voudrias EA. Laboratory scale bioremediation of petroleum-contaminated soil by indigenous microorganisms and added Pseudomonas aeruginosa strain Spet. Bioresource Technology. 2010;101(16):6545-6552.
Atlas RM. Microbial ecology: fundamentals and applications. Pearson Education India; 1998.
Mueller RJ. Biological degradation of synthetic polyesters—Enzymes as potential catalysts for polyester recycling. Process Biochemistry. 2006;41(10):2124-2128.
Tokiwa Y, Calabia BP. Review degradation of microbial polyesters. Biotechnology Letters. 2004;26(15):1181-1189.
Jendrossek D. Peculiarities of PHA granules preparation and PHA depolymerase activity determination. Applied Microbiology and Biotechnology. 2007;74(6):1186.
Chen GQ. A microbial polyhydroxyalkanoates (PHA) based bio-and materials industry. Chemical Society Reviews. 2009;38(8):2434-2446.
Braunegg G, Koller M, Varila P, Kutschera C, Bona R, Hermann C, Pereira L. Production of plastics from waste derived from agrofood industry. CRC Press, Taylor & Francis Group, Boca Raton, Florida, USA. 2007;119-135.
Mohanty AK, Misra M, Drzal LT. Sustainable bio-composites from renewable resources: opportunities and challenges in the green materials world. Journal of Polymers and the Environment. 2002;10(1-2):19-26.
Braunegg G, Lefebvre G, Genser KF. Polyhydroxyalkanoates, biopolyesters from renewable resources: physiological and engineering aspects. Journal of Biotechnology. 1998;65(2-3):127-161.
Woese CR. Bacterial evolution. Microbial Revolution. 1987;57:221-71.
Garrity G. Bergeys Mannual of systemic bacteriology. London. Springer; 2005.
Schut F, de Vries EJ, Gottschal JC, Robertson BR, Harder W, Prins RA, Button DC. Isolation of typical marine bacteria by dilution culture, groth, maintenance and characteristics of isolates under laboratory conditions. Applied Environmental Microbiology. 1993;59(7): 2150-2160.
Arun A, Arthi R, Shanmugabalaji V, Eyini M. Microbial production of poly-β-hydroxybutyrate by marine microbes isolated from various marine environments. Bioresource Technology. 2009;100(7):2320-2323.
Ibrahim MH, Steinbüchel A. Poly (3-hydroxybutyrate) production from glycerol by Zobellella denitrificans MW1 via high-cell-density fed-batch fermentation and simplified solvent extraction. Applied Environmental Microbiology. 2009;75(19):6222-6231.
Hungund B, Shyama VS, Patwardhan P, Saleh AM. Production of polyhydroxyalkanoate from Paenibacillus durus BV-1 isolated from oil mill soil. Journal of Microbiology, Biochemistry Technology. 2013;5:013- 017.
Lee EY, Choi CY. Structural identification of polyhydroxyalkanoic acid (PHA) containing 4- hydroxyalkanoic acids by gas chromatography – mass spectrometry (GC-MS) and its applications to bacteria screening. Biotechnology Research. 1997;11:167-171.
Dawes EA. Polyhydroxybutyrate: an intriguing biopolymer. Bioscience Report. 1988;8:537-547.
Anonymous. Biodegradable plastics, Developments and Environmental impacts. Nolan – ITU – Pvt Ltd, prepared in association with Excelplas Australia; 2002.
Brião VB, Tavares CR. Effluent generation by the dairy industry: preventive attitudes and opportunities. Brazilian Journal of Chemical Engineering. 2007;24(4):487-497.
Chawla A, Chawla N, Pant Y, Kandhari P. Milk and dairy products in India–production, consumption and exports. Hindustan Studies and Services Limited, Bhopal, India; 2009.
Sharma VP, Gulati A. Trade liberalization, market reforms and competitiveness of Indian dairy sector (No. 596-2016-40027); 2003.
Kolhe AS, Pawar VP. Physico-chemical analysis of effluents from dairy industry. Recent Research in Science and Technology. 2011;3(5).
Garrido JM, Omil F, Arrojo B, Mendez R, Lema JM. Carbon and nitrogen removal from a wastewater of an industrial dairy laboratory with a coupled anaerobic filter-sequencing batch reactor system. Water Science and Technology. 2001;43(3):249-256.
Zamora AF, Lit MAL. Biodegradation of effluents from fish canning factory and dairy products processing plant. In Environmental Biotechnology. Springer, Dordrecht. 1995;481-490.
Ojo OA. Petroleum-hydrocarbon utilization by native bacterial population from a wastewater canal Southwest Nigeria. African Journal of Biotechnology. 2006;5(4):333- 337.
Grothe E, Moo-Young M, Chisti Y. Fermentation optimization for the production of poly (β-hydroxybutyric acid) microbial thermoplastic. Enzyme and Microbial Technology. 1999;25(1-2):132-141.
Goff M, Ward PG, O’Connor KE. Improvement of the conversion of polystyrene to polyhydroxyalkanoate through the manipulation of the microbial aspect of the process: a nitrogen feeding strategy for bacterial cells in a stirred tank reactor. Journal of Biotechnology. 2007;132(3):283-286.
Cesário MT, Raposo RS, de Almeida MCM, van Keulen F, Ferreira BS, da Fonseca MMR. Enhanced bioproduction of poly-3-hydroxybutyrate from wheat straw lignocellulosic hydrolysates. New Biotechnology. 2014;31(1):104-113.