EVALUATING THE ANTIBACTERIAL ACTIVITY OF MORINDA MORINDOIDES EXTRACTS ON THE IN VITRO GROWTH OF THE CLINICAL STRAINS OF Shigella sp.
KOFFI ALLALI EUGÈNE *
College of Agroforestry, Agrovalorisation Laboratory, Department of Biochemistry and Microbiology, Jean Lorougnon GUEDE University, P.O Box 150 Daloa, Côte d'Ivoire.
DOSSO MAMADOU
Biological Sciences, College of Biochemistry and Genetics, Peleforo Gon Coulibaly University, P.O Box 1328 Korhogo, Côte d'Ivoire.
ABBA P. OBOUAYEBA
College of Agroforestry, Agrovalorisation Laboratory, Department of Biochemistry and Microbiology, Jean Lorougnon GUEDE University, P.O Box 150 Daloa, Côte d'Ivoire.
DJAKO S. T. AKRÉ
College of Agroforestry, Agrovalorisation Laboratory, Department of Biochemistry and Microbiology, Jean Lorougnon GUEDE University, P.O Box 150 Daloa, Côte d'Ivoire.
N’GUESSAN KOUADIO MICHAEL
College of Agroforestry, Agrovalorisation Laboratory, Department of Biochemistry and Microbiology, Jean Lorougnon GUEDE University, P.O Box 150 Daloa, Côte d'Ivoire.
A. SAKIRIGUI
Laboratory of Pharmacognosy and Essential Oils, Faculty of Sciences and Techniques/University of Abomey-Calavi (UAC) Cotonou, Republic of Benin and Aromatic, Food and Medicinal Plants Research Laboratory, Faculty of Sciences and Techniques, UNSTIM/Abomey Republic of Benin, République du Bénin.
EHOUMAN MOCKET ADOLPHE
Olopam Pharma and Research & Development, Côte d'Ivoire.
A. B. ACKAH JACQUES AUGUSTE
College of Agroforestry, Agrovalorisation Laboratory, Department of Biochemistry and Microbiology, Jean Lorougnon GUEDE University, P.O Box 150 Daloa, Côte d'Ivoire.
*Author to whom correspondence should be addressed.
Abstract
For a couple of years, strains of Shigella resistant to aminopenicillins and cotrimoxazole have developed and have become predominant.
The purpose of paper is to show the social contribution in the care of gastroenteritis. As such, the antibacterial activity of the total aqueous and 70% ethanolic extracts of the leaves of Morinda morindoides, a plant from the Ivorian pharmacopoeia was evaluated.
In this paper, bacteriological tests were carried out on two pathogenic strains of Shigella sp. (EEQ and 1055).
The methods of diffusion, either in agar medium or in liquid, were used for the search for Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC).
The results show that both extracts tested have in vitro antibacterial bactericidal efficiency on both Shigella sp. strains. However, the 70% ethanolic extract which is twice as effective as the total aqueous extract is more active against strains of Shigella Sp tested, with a MBC of 25 mg/mL.
The finding suggest that antibacterial properties of this plant could also useful for caring shigellosis.
Keywords: Morinda morindoides, aqueous extract, ethanolic extract, bacterial strains, Shigella sp., MBC, MIC
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Mpiana PT, Ngbolua KN, Tshibangu, DST, Kilembe JT, Gbolo BZ, Mwanangombo DT, Clement L, Inkoto Lengbiye EM, Mbadiko CM, Matondo, A, Bongo GN, Dorothée D. Tshilanda. Aloe vera (L.) Burm. F. as a Potential Anti-COVID-19 Plant: A Minireview of Its Antiviral Activity. EJMP. 2020;31(8):86-93.
World Health Organization (WHO). WHO for traditional medicine strategy 2014-2023. 2013;78.
Available:https://apps.who.int/iris/rest/bitstreams/434690/retrieve
World Health Organization. Traditional medicine. Fact sheet Number 134. Available:http://www.who.int/mediacentre/factsheets/fs134/en/
Accessed 2 February 2015.
Inkoto CL, Kayembe JPK, Mpiana PT, Ngbolua KN. A review on the Phytochemistry and Pharmacological properties of Picralima nitida Durand and H. (Apocynaceae family): A potential antiCovid-19 medicinal plant species. Emer Life Sci Res. 2020a 6(1):64-75.
Inkoto CL, Masengo CA, Falanga CM, Membo WMB, Amogu JJD, Mahendra ISM, Kayembe JPK, Mpiana PT, Ngbolua KN. A mini-review on the phytochemistry and pharmacology of the plant Carica papaya L. (Caricaceae). Britain International of Exact Sciences. 2020b;2(3): 663-675.
Nkasa HL, Clément Inkoto CL, Muzomwe MJC, Masengo EI, Mumba CM, Mulenga CM, Taba KM. Phytochemical screening and antibacterial activity of phytomedecine mathesia, a drug use against buruli ulcer in republic Democratic of the Congo (Drc), European Journal of • Pharmaceutical And Medical Research (EJPMR). 2020;7(2):52-56.
Sylvie Bolisomi Bongili, Clément Liyongo Inkoto, Néhémie Yamfu Bindanda, Albert Bushabu Kongo, 2Blaise Mbembo wa Mbembo, 3Oscar IsekatsilaN’singa, Andrien Masunda Tuwisana, Pius Tshimankinda Mpiana and Koto-te-Nyiwa Ngbolua. Morinda morindoides (Backer) Milne-Redh (Rubiaceae): Phytochemistry, pharmacological activities and future direction: A mini-review. International Journal of Science Academic Research. 2020;01(09):663-668.
Sidio Serge Roland, N’guessan Koffi, Ethnobotanical study of medicinal plants used to combat gastroenterological disorders in populations of the Department of Gagnoa, in Central Western Côte d’Ivoire. European Scientific Journal. 2019;15(36):320 – 343.
Yasinjan Hashim, Kazufumi Toume, Shusaku Mizukami, Yue-Wei Ge, Mayumi Taniguchi, Awet Alem Teklemichael, Nguyen Tien Huy, Joseph M. Bodi, Kenji Hirayama, Katsuko Komatsu. Phenylpropanoid conjugated iridoids with anti-malarial activity from the leaves of Morinda morindoides. Journal of Natural Medicines; 2021.
DOI: 10.1007/s11418-021-01541-x
Cimanga RK, Kambu K, Tona L, Hermans N, Apers S, Totte J, Pieters L, Vlietinck AJ. Cytotoxicity and in vitro susceptibility of Entamoeba histolytica to Morinda morindoides leaf extracts and its isolated constituents. Journal of Ethnopharmacology. 2006a;107:83-90.
DOI:https://doi.org/10.1016/j.jep.2006.02.010
Cimanga K, Kambu K, Tona L, Hermans N, Apers S, Totté J, Pieters L, Vlietinck AJ. Antiamoebic activity of iridoids from Morinda morindoides leaves. Planta Med. 2006;72:751–753.
DOI:https://doi.org/10.1055/s-2006-931581
Moroh JLA, Bahi C, Karamoko D, Fleury Y, Labia R, Leguerinel I, Coulibaly A. Highlighting of the antimicrobial activities of extracts of Morinda morindoides, a medicinal plant used in West and Central Africa. SSRG International Journal of Medical Science. 2016; 3(12):18-24.
Moroh JLA, Bahi C, Djé K, Loukou YG, Guédé Guina F. Study of the antibacterial activity of Acetatic Extract (EAC) of Morinda morindoides (Baker) MiilneRedh (Rubiaceae) on the in vitro growth of strains of Escherichia coli. Bulletin of the Royal Society of Sciences of Liège. 2008;77: 44-61.
Kouame BF. Antibacterial activity of BGG, a naturally occurring antidiarrhoeal substance against Samonella O.M.A. (Total aqueous and ethanolic extract 70%). DEA thesis. Biotechnologies and food sciences option Pharmacology of Natural Substances. University of Cocody, UFR Biosciences, Abidjan, Ivory Coast. 2006;47.
Touré A, Ouattara K, Ouattara A, Coulibaly A. Antimicrobial tests of soap based on ethanolic extract of leaves of Morinda morindoides (Morinda, Rubiaceae) on in vitro growth of germs involved in skin infections. Phytothérapie. 2017;15:197–202.
Moroh JLA, Fleury Y, Coulibaly A, Labia R, Leguérinel I. Chemo-diversity of antibacterial anthraquinones from the Roots of Morinda morindoides. The Natural Products Journal. 2019;9(4): 256-261.
Koffi AE, Dosso M, Libra M. A, Ehouman MA, Yapi HF, Djaman JA. Phytochemical and comparative studies of two types of ethanolic extracts of Morinda morindoides (Baker) Milne-Redhead (Rubiaceae) on the in vitro growth of cholera vibrio strains. Interdisciplinary Journal of Scientific Research. 2020;1: 1-7.
Tra- Bi OI, Djyh BN, Bahi C., Meite S. and N'gue San JD. A study of the hepatic tolerance of the ethyl acetate fraction of Morinda morindoides (Baker) Milne-Redhead (Rubiaceae) Extract in Rabbit. Tropical Journal of Pharmaceutical Research. 2010;9(6):573-579.
Tra-BI O. I., Djyh B. N., Yeo D., Ito M. P. Mrs Tebele, Djaman A. J. N’guessan J. D. Renal Tolerance Study of the Ethyl Acetate Fraction of Morinda morindoides (Baker) Milne-Redhead (Rubiaceae) Extract in Rabbit. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2011;2: 530-540.
Irene Stefanini, Martina Boni, Paola Silvaplana, Paola Lovera, Stefania Pelassa, Giuseppe De Renzi, Barbara Mognetti. Antimicrobial resistance, an update from the Ward: Increased incidence of new potential pathogens and site of infection-specific antibacterial resistances. Antibiotics. 2020;9: 0631 : 2 of 14.
Liliana Serwecińska. Antimicrobials and antibiotic-resistant bacteria: A risk to the environment and to public health. Water. 2020;12(12):3313: 2 of 17.
DOI:https://doi.org/10.3390/w12123313
Divya Ganesana, Sanjukta Sen Guptab, Dominique Legros. Cholera surveillance and estimation of burden of cholera. Vaccine. 2020 ;38:A13–A17.
DOI:https://doi.org/10.1016/j.vaccine.2019.07.036
Aubry P, Gaüzère B. Diarrhées infectieuses, Actualités 2019, Centre René Labusquière, Institut de Médecine Tropicale, Université de Bordeaux, 33076 Bordeaux (France). 2019;8.
Katarzyna Stażyk, Róża Krycińska, Czepiel Jacek, Aleksander Garlicki, Grażyna Biesiada. Diarrhea caused by Shigella flexneri in patients with primary HIV infection International Journal of STD & AIDS. 2019;30(8):814-816.
DOI: 10.1177/0956462418813060
Agence française de sécurité sanitaire des produits de santé (Afssaps) .Recommandations - Traitement antibiotique des gastro-entérites à Shigella sonnei 1538-1545 -01/03/08
Available:https://www.em-consulte.com/article/102112/recommandations-traitement-antibiotique-des-gastro
Doi : PM-12-2004-33-21-0755-4982-101019-ART15
Naeemah Z Logan, MD, Beth E Karp, DVM, MPH, Kaitlin A Tagg, PhD, Claire Burns-Lynch, MPH, Jessica Chen, PhD, Amanda Garcia-Williams, PhD, Zachary A Marsh, MPH, Kevin O’Laughlin, MD, Ian D Plumb, MBBS, MSc, Morgan N Schroeder, MPH ... and al. Increase in Multidrug Resistance (2011–2018) and the Emergence of Extensive Drug Resistance (2020) in shigella Sonnei in the United States. Open Forum Infectious Diseases. 2020;7(Suppl 1):S195.
DOI:https://doi.org/10.1093/ofid/ofaa439.440
Bauer A.W., Kirby W.M.M., Sherris T.C. & Truck M. Antibiotic susceptibility testing by a standardized single disc method, American Journal of Clinical Pathology. 1966;45:493 - 496.
Obou Constantin Okou, Sopie Edwige-Salomé Yapo, Kouassi Clément Kouassi, N’Guessan Romain Komenan, Sylvia Venerer Monthaut, Adou Koffi Mathieu KRA, Allico Joseph Djaman. Évaluation de l’activité antibactérienne des extraits de fruits de Solanum torvum Swartz (Solanaceae) sur la croissance in vitro de sept (07) souches d’entérobactéries de différents profils (résistantes ou sensibles. Int. J. Biol. Chem. Sci. 2019;13(3):1510-1526.
Esseh K, Afanyibo YG, Ahama-Esseh KYS, Idoh K, Koudouvo K, Agbonon A, Gbeassor M. Screening phytochimique, étude toxicologique, évaluation des activités antiplasmodiale et antiradicalaire de la tige feuillée de Senna occidentalis Linn (Fabaceae) European Scientific Journal. 2019 ;15(6 ):411-433.
Lango-Yaya Ernest, Worowounga Xavier, Issa-Madongo Mathurin, Namkona Armel-Frederic, Saravolia Marinette, Rafai Donatien Clotaire and Syssa-Magalé Jean-Laurent. Evaluation in vitro de l’activité antibactérienne des extrais méthanoliques des écorces de racines de Paullinia pinnata et des feuilles de Petaclethra macrophylla. Journal of Applied Biosciences. 2020;147: 15151 –15158.
Toty AA, Guessennd N, Bahi C, Kra AM, Otokore DA, Dosso M. In-vitro evaluation of the antibacterial activity of the aqueous extract of the trunk bark of Harungana madagascariensis on the growth of multi-resistant strains. Bulletin of the Royal Society of Sciences of Liège. 2013;82:12-21.
Okou OC, Yapo S, Kporou KE E, Baibo G, L, Monthaut S, Djaman AJ. Evaluation of the antibacterial activity of extracts of leaves of Solanum torvum Swartz (Solanaceae) on in vitro growth 3 strains of Enterobacteriaceae; Journal of Applied Biosciences. 2018; 122: 12287-12295.
Marmonier AA. Agar diffusion technique: Disc method. In Medical Bacteriology, Usual Techniques. 1990;7:237-244.
Zirihi GN, Kra AKM, Guédé-Guina F. Evaluation of the antifungal activity of Microglossa pyrifolia (Lamarck) O. Kantze (Asteraceae) "PYMI" on the in vitro growth of Candida albicans, Revue de Médecine et Pharmacie Afrique. 2003;17:11-18.
Koffi AE, Yapi HF, Bahi C, Guessend KN, Djaman JA, Guede-Guina F. Activity of Morinda morindoides on the in vitro growth of the cholera vibrio: experiment in Ivory Coast. Tropical Medicine. 2010;70: 53-56.
Aubry P, Gaüzère B. Shigellosis 2016, Center René Labusquière, Institute of Tropical Medicine, University of Bordeaux, 33076 Bordeaux (France). 2016;4.
Available:http://medecinetropicale.free.fr/cours/shigellose.pdf
Ibrahim L. Evaluation of the antibacterial properties, in vitro, of extracts of leaves of Argemone mexicana L. (Papaveraceae), PhD thesis in Pharmacy, University of Ouagadougou. Burkina Faso. 2010;76.
Thangara JHS, Adjei O, Allen BW, Portaels F. In vitro activity of ciprofloxacin, sparfloxacin, ofloxacin, amikacin and rifampicin against Ghanian isolates of Mycobacterium ulcerans; Journal Antimicrob Agents Chemother. 2000;45(2): 231-233.
Available:www.beep.ird.fr/collect/uouaga/index/assoc/M13090.dir/M13090.pdf
Moroh JLA. Bacterial resistance and antimicrobial phytomolecules from Morinda morindoides, doctoral thesis, faculty of microbiology-biochemistry, University Felix Houphouët Boigny (Abidjan). 2013;213 .